• Protein reagents - Protein A and Protein G
  • Only commercial production that is 100%-free of all animal and plant extracts
  • Produced using glucose/salts minimal media
  • High quality and documented lot-to-lot reproducibility
  • Very competitive bulk-order pricing
• Variant gene synthesis/protein expression and purification
  • Optimization of expression and protein yield
  • Custom expression in bacterial, yeast, and mammalian systems
  • Western blot analysis
  • Maldi-MS
• Small scale protein production
  • Optimize expression conditions
  • Evaluate multiple variants for expression and activity
  • Expression conditions for seamless scale-up to large scale production
• Large scale protein production:
  • 10L - 130L batch sizes
  • Up to "kilogram"-scale capacity
• Bacterial expression with proprietary CleanGenome® strains
  • High expression with optimized conditions for each protein
  • Removed E coli genes that gluconate proteins (BL-21 gluconates)
  • Extremely tight and proprietary regulation of transcription, aids in expression of difficult (i.e. toxic) proteins
  • CleanGenome® strains contain *no* IS elements, transposons or prophage sequences
  • Extremely reduced protease & nuclease activity
• Yeast expression
  • Pichia – variant human proteins used as controls in therapeutic clinical trials
  • Saccharomyces - alternative post-translational processing
• Recombinant monoclonal antibody expression in mammalian cell lines including CHO and HEK293
• Single Chain Antibodies (scFV)
  • Extensive work on multiple DARPA scFv projects.
  • Production of thousands of scFV variants
  • Development and optimization of binding specificities
• Custom protein modifications (i.e. biotinylation, cross-linking, fluorophores, etc.)
  • Assay development
  • ELISA & MSD-ECL binding assays
  • Develop and optimize assays de novo
  • Convert existing assays between ELISA & MSD-ECL platforms
  • Assay optimization for sensitivity, specificity and reproducibility
  • FACS (Fluorescence-activated cell sorting) assays
  • Custom-labeled antibodies
    • Biotinylation
    • ECL tags
    • Fluorescent labels
  • Cell-based assays
• Gene cloning and expression
  • Confirmation of vector integration
  • Real-time PCR

Gary Baisa, Ph.D.

Lead Scientist for Yeast Protein Expression

Gary.Baisa@lyticsolutions.com

Gary join Lytic solutions in 2017 bringing expertise in microbiology, molecular biology, and plant biology. Gary earned his Ph.D. in Pathogenic Microbiology from UW-Madison.